The ability to alter the genetic composition of living cells has revolutionized biology. Scientific advances, from treating genetic disorders through gene therapy to reprograming skin cells into ...

Striking the Right Balance Becomes More Challenging If Co-Transfection Scenarios Are Planned Transfection technology awaits no rallying cry. It hastens from siege to siege, attacking cellular bastions ...

This protocol outlines steps for optimizing the transfection of adherent primary mammalian cells using the readily available off-the-shelf cationic polymer, 25-kDa branched polyethylenimine (bPEI25).

The transfection of genes and the delivery of cell membrane–impermeable biomolecules—potentially drugs—into mammalian cells are among the most important research tools in modern molecular biology. A ...

Transfection alters the genetic makeup of eukaryotic cells by introducing foreign nucleic acids, including DNA, RNA, and small noncoding RNAs such as siRNA, shRNA, and miRNA. Scientists use ...

Transfection is a crucial non-viral technique for introducing DNA or RNA into cells, yet achieving high efficiency and consistency can be challenging, particularly with difficult cell types.

Transient Transfection Can Reward Impatience by Quickly Providing High Protein Titers without Penalizing Production Down the Line Transient transfection has become the go-to method for obtaining gram ...